Fig 9.

(A) The effects of IRF7 variants on the IRF3 activity. 293T cells were transfected with IRF7, 189N, 189C, or GFP-IRF3 along with ISRE-Luc as indicated. pRL-SV40 was used as a control. At 24 h after transfection, cell lysates were assayed for luciferase activities. Data are a representative of three independent experiments with triplicate samples. (B) Expression of IRF7 variants and IRF3. Cell lysates in panel A were detected by Western blotting with antibodies against Myc, GFP, and β-actin. (C) IRF7 variants interact with IRF3. 293T cells were transfected with plasmids encoding GFP-IRF3 (lanes 1, 2, 3, and 4), Myc-Flag-IRF7 (lane 2), Myc-Flag-189N (lane 3), and Myc-Flag-189C (lane 4). At 24 h after transfection, cell lysates were immunoprecipitated with anti-Myc or anti-GFP antibody. Immunoprecipitates and aliquots of cell lysates were then subjected to Western blot analysis.