Fig 3.

Lipid raft disruption through cholesterol depletion inhibits PI3K DRM localization. Rat-1 (A) or C666 (B) cells expressing LMP1 were serum starved for 1 h and then treated with MβCD for 30 min at 37°C. DRMs were then isolated, and equivalent amounts by volume were analyzed by immunoblotting for PI3K, LMP1, and flotillin-2 (Flot-2) levels. The band intensities of PI3K, LMP1, and flotillin-2 were determined using ImageJ software and are represented relative to the untreated control level. (C) The results of three independent experiments were graphed as mean averages with standard errors of the mean. Statistical significance was determined by using the paired two-tailed Student t test.