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Effects of substitutions of TM1. (A) Substitution of TM1 with the type I signal from β-lactamase in the H background (mutant 1B) and M background (mutant MB). (B) Marked transdominant negative effect of mutant 1B on the release of coexpressed S protein. ly, cell lysate; cs, culture supernatant; m, mock transfected.
