Fig 4.

Detection of total RABV N RNA in different tissues after i.m. administration of TriGAS. Groups of mice were injected into the gastrocnemius muscle with 10⁵ FFU (A) or 10⁷ FFU (B) of TriGAS. At the indicated times after inoculation, mice were euthanized and RNA was isolated from muscle, draining lymph node, spleen, brain, and spinal cord, and the amounts of total RABV N RNA were determined by quantitative reverse transcription-PCR (qRT-PCR) as described in Materials and Methods. Presented are the mean copy numbers (± SE) of RABV N RNA per 1,000 copies of L13 mRNA. Significant differences in expression of RABV RNA are indicated as follows: *, P < 0.05; **, P < 0.01; ***, P < 0.001.