Fig 2.

Vaccine-induced T-cell responses. All the data were obtained from PBMCs harvested at 3 weeks after the last immunization (week 27). All the data from animals protected from SIV_mac251 acquisition are depicted with open symbols. IFN-γ-secreting cells measured by ELISpot in response to SIV Env (A) or Gag (B). Results are shown as the number of spots per 10⁶ cells, and each symbol represents one animal. Lymphoproliferative responses (LPR) measured by thymidine incorporation to SIV Env (C) or Gag (D). The horizontal bars in the figures represent the average and standard error for the vaccinated and control animals. Gating strategy for CD4⁺ and CD8⁺ CFSE⁺ memory (CD95⁺) T cells (E). (F to I) Percentage of CD95⁺ CFSE dim or negative CD8⁺ or CD4⁺ T cells that proliferate following Env or Gag stimulation.