Fig 1.

BMDC, sDC, and human DC infectivity rates. DCs infected with influenza A/California/7/2009 (A), influenza A/New Caledonia/20/1999 (B), and influenza A/PR/8/34 (C) assayed 8 h after infection for viral NP expression with flow cytometry (***, P < 0.005). NP expression reaches a plateau 6 to 8 h after infection (data not shown). (D) Human and murine DC viral gene expression rate assayed by FISH (influenza NA assayed) and single-cell qPCR (influenza NS1 assayed) measured 8 h and 6 h postinfection, respectively. Results shown in panels A to D were obtained using cells from four different donors.