Table 4.

Biophysical data of the peptide analogs

Peptidea	Benignb		50% TFEc		tR (min)e
	[θ]222	% helixd	[θ]222	% helixd
GBVA10	−1,700	5.59	−15,400	50.57	37.47
GBVA10-1(L9K)	−1,700	5.59	−8,600	28.33	31.47
GBVA10-2(L9S)	−1,250	4.12	−6,650	21.88	32.59
GBVA10-3(L9A)	−950	3.12	−8,750	28.80	33.57
GBVA10-4(L9V)	−2,250	7.45	−14,550	47.79	35.72
GBVA10-5(G6A)	−3,200	10.60	−20,000	65.73	40.85
GBVA10-6(G6V)	−3,550	11.61	−25,300	83.10	43.88
GBVA10-7(G6L)	−4,050	13.39	−26,050	85.84	44.94
GBVA10-7(G6L)	−4,050	13.39	−26,050	85.84	44.94
GBVA10-8(G6L/P16L)	−6,400	21.14	−30,400	100.00	51.59
GBVA10-9(P16L)	−2,050	6.79	−24,900	81.91	43.72
GBVA10-10(L9I)	−2,900	9.56	−15,550	51.06	37.46
GBVA10-11(L9I/L13I)	−2,550	8.32	−13,200	43.37	36.46
GBVA10-12(L5I/L9I/L13I)	−4,400	14.48	−11,350	37.31	36.09
GBVA10-13(C1K/Y8K/T15K/T18K)	−750	2.50	−9,900	32.47	28.97
GBVA10-14(C1K/Y8K/T15K)	−450	1.52	−12,000	39.41	30.89
GBVA10-15(Y8K/T15K)	−1,450	4.74	−20,300	66.68	33.67
GBVA10-16(Y8K)	−2,900	9.52	−15,250	50.20	34.53
GBVA10-17(C1E/Y8E/T15E)	−2,350	7.71	−17,950	58.96	36.28
GBVA10-18(C1E/Y8E/T15E/T18E)	−4,300	14.16	−13,000	42.80	36.44
GBVA10-19(Y8E/T15E)	−4,600	15.16	−18,600	61.23	37.16
GBVA10-20(Y8E)	−2,200	7.24	−18,250	59.94	37.33

^aPeptides are ordered by relative hydrophobicity during RP-HPLC at 25°C.

^bThe mean residue molar ellipticities, [θ]₂₂₂ (degree · cm² · dmol⁻¹) at a wavelength of 222 nm, were measured at 25°C in KP buffer (100 mM KCl, 50 mM PO₄; pH 7.0).

^cThe mean residue molar ellipticities, [θ]₂₂₂ (degree · cm² · dmol⁻¹) at a wavelength of 222 nm, were measured at 25°C in KP buffer diluted 1:1 (vol/vol) with TFE.

^dThe helical content (percentage) of a peptide relative to the molar ellipticity value of peptide G6L/P16L in 50% TFE.

^et_R, retention time at 5°C and 80°C during the RP-HPLC temperature profiling.