Table 1.
Participant | Total no. of sequences |
env sequence |
pol (RT) sequence |
||||||
---|---|---|---|---|---|---|---|---|---|
No. | % |
No. | % |
||||||
Pre-ART | Sputum | Concordant | Pre-ART | Sputum | Concordant | ||||
B1 | 324 | 182 | 9 | 33 | 41 | 142 | 8 | 35 | 61 |
B2 | 179 | 100 | 11 | 41 | 49 | 79 | 22 | 38 | 97 |
F1 | 269 | 120 | 10 | 29 | 64 | 149 | 15 | 35 | 60 |
L1 | 295 | 104 | 7 | 26 | 44 | 191 | 13 | 21 | 51 |
R1 | 400 | 210 | 10 | 34 | 37 | 190 | 12 | 33 | 56 |
V1 | 189 | 114 | 11 | 43 | 61 | 75 | 17 | 43 | 93 |
Median | 282 | 117 | 10 | 32 | 47 | 145.5 | 14 | 34 | 61 |
Total | 1,656 | 830 | 826 |
Concordant sequences are defined by amplification of both env and pol in the same first-round PCR. Because the region of env sequenced is longer and more variable than the RT sequence, more env sequencing reactions failed, resulting in a greater percentage of concordant RT sequences.