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IgG1 and IgG2a levels in vaccinated mice. Levels of HA-specific IgG1 (red line) and IgG2a (black line) from intramuscularly (i.m., top) and intranasally (i.n., bottom) vaccinated animals measured by ELISA. Equivalent amounts of sera from 5 animals treated with the same adjuvant were pooled together before the antibody quantification.
