Fig 9.

Survival and weight loss of vaccinated animals. Mice (n = 5) were vaccinated i.m. (A, top) or i.n. (B, bottom) with inactivated influenza A/Puerto Rico/8/34 virus supplemented with squalene-based oil in water emulsion (AddaVax; 1:1 [vol/vol]; purple line; only for the i.m. vaccinated animals), pIC (4 μg; orange line), or the IVT DI (4 μg; blue line) or were mock vaccinated (black line) or vaccinated with the inactivated vaccine with no adjuvant (gray line). In the case of the i.n. vaccinations, an additional group of mice that were treated with 4 μg of the IVT DI and did not receive the vaccine (pale blue line) was included. Twenty-three days after vaccination, animals were challenged with 5,000 (i.m.) or 1,000 (i.n.) PFU of influenza A/Puerto Rico/8/34 virus. The weight of the animals was recorded over 15 days. Animals with >25% weight loss were euthanized. Left panels show the survival rates, while right panels show the percentage of initial weight throughout the course of the infection. Error bars represent standard deviations from five mice.