Fig 2.

LPS, but not HSV-2, triggers DC maturation. (A and B) Immature moDCs were exposed to LPS (1 μg/ml) or HSV-2(G) (live or UV inactivated) (MOI = 5 PFU/cell) for 1 h at 37°C. The cells were analyzed by flow cytometry for surface marker CD83 expression at 4 h posttreatment. The data are representative dot plots (A) or the mean percent % CD83⁺ cells + the SEM from six independent experiments (B). (C and D) Further analysis of CD83 expression within infected (GFP⁺; darkest two segments) or bystander (GFP⁻; lightest two segments) populations following exposure of DCs to LPS or HSV-2(333)ZAG (MOI = 0.01 to 10 PFU/cell as indicated) at 4 and 8 h postexposure (C and D, respectively). The results are presented as the percentage of 10,000 live events acquired and are means obtained from four independent experiments. Asterisks indicate significant differences relative to mock-treated DCs (P = 0.05).