Fig 8.

Live HSV-2 triggers the release of TNF-α and IL-6, whereas UV-inactivated virus triggers an IFN response. (A) Immature moDCs were mock infected, treated with LPS (1 μg/ml) or staurosporine (1 μM), or infected with live or UV-inactivated HSV-2(G) (MOI = 5 PFU/cell), and culture supernatants were harvested at 24 h p.i. and analyzed by Luminex for cytokine levels. The results are means + the SEM obtained from duplicate wells from a minimum of nine independent experiments. (B and C) Immature moDCs were exposed to the same stimuli and, 24 h postexposure, culture supernatants were assessed for the release of IFN-β by ELISA (B) or the release of IFN-γ by Luminex (C). (D) The cells were also lysed at the indicated times for the measurement of IFN-β gene expression by qRT-PCR. The data are presented as pg/ml released (A, B, and C) and gene expression (relative to the housekeeping α-tubulin gene) (D). The data are means + the SEM from three independent experiments. The asterisks indicate significant differences relative to mock-treated DCs (P < 0.05).