Fig 3.

Inhibition of endogenous reverse transcription by ectopically expressed APOBEC3G. (Top) Immunoblotting was used to compare wild-type and ΔVif virions derived from infected primary CD4⁺ T cells (lanes 1 and 2, respectively), with virions generated by transfection of 293T cells with pIIIB Δvif and increasing quantities of pcDNA3.1 A3G (lanes 3 to 8, A3G/provirus transfection ratios of 0:1, 1:81, 1:27, 1:9, 1:3, and 1:1, respectively). (Bottom) Virions analyzed in lanes 3 to 8 of panel A were assessed in ERT assays. Total DNA was harvested at the indicated times, and levels of strong stop cDNA were measured using qPCR.