Fig 4.

Impact of APOBEC3G on length of HIV-1 cDNA synthesized by endogenous reverse transcription. Virions characterized for Fig. 3 and generated by transfection of 293T cells with pcDNA3.1 A3G and pIIIB Δvif (0:1, 1:27, and 1:1 ratios) were purified and subjected to ERT reactions. Nucleic acids were extracted at the indicated times, and cDNA products were tailed, amplified, cloned, and sequenced. The lengths of between 22 and 46 reverse transcripts per time point and A3G concentration are shown. cDNA length is plotted on the ordinate, with the abscissa corresponding to individual cDNAs sorted by length. Each graph corresponds to one A3G/provirus ratio and demonstrates progression of reverse transcription over four time points (30, 60, 120, and 240 min).