Fig 2.

IRF-3 mediates IFN-β transcriptional induction, enhances neuronal survival, and suppresses infectious virion production in BE(2)-C/m cells in response to WEEV infection. (A) BE(2)-C/m cells stably expressing control vector or dnIRF-3 construct were mock infected or infected with WEEV at an MOI of 1 for 20 h, and IFN-β transcript levels were measured by qRT-PCR. (B and C) BE(2)-C/m cells expressing the vectors described in panel A were infected with WEEV at the indicated MOI and both the percent viability relative to mock-infected controls (B) and infectious viral titers in tissue culture supernatants (C) were measured at 48 hpi. Similar differences in viral titers were seen when tissue culture supernatants were examined at 20 hpi (data not shown).