Fig 9.
WEEV-mediated suppression of host translation is reduced in neuronal cells. (A) Control BHK-21 (upper images) or BE(2)-C/m (lower images) were infected with WEEV at an MOI of 10 and analyzed by immunofluorescence microscopy at the indicated times postinfection. Representative overlaid images from one of two independent experiments are shown, where blue indicates DAPI-stained nuclei and green indicates WEEV-infected cells. Scale bars, 25 μm. (B) Control BHK-21 (lanes 1 to 6) or BE(2)-C/m (lanes 7 to 10) were infected with WEEV at an MOI of 10 and labeled with 50 μCi/ml [35S]methionine-cysteine for 30 min prior to harvesting at 3, 6, 9, and 12 hpi, and lysates were analyzed by SDS-PAGE and fluorography. Mock-infected (lane 1) and cycloheximide-treated (lane 2) BHK-21 cells are shown as representative controls. Molecular weight (MW) markers (in thousands) are shown on the left, and presumptive WEEV capsid protein is indicated by the arrow. (C) Translation of the cellular protein at ∼45 kDa, marked by the asterisk in panel B, was quantitated by densitometry for BHK-21 and BE(2)-C/m cells, and results are expressed as the percentage of uninfected control cells.