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. 2013 Feb;87(3):1720–1732. doi: 10.1128/JVI.01955-12

Fig 5.

Fig 5

Colocalization and interaction of pUL51 with the terminase subunits pUL56 and pUL89. (A) Cells infected with HD-UL51-NHA were probed on day 3 p.i. with antibodies against the HA tag and pUL56 (upper panel) or pUL89 (lower panel) and then analyzed by confocal laser scanning microscopy. Bar, 5 μm. (B) HFF infected with HD-UL51-SF or the parental virus HD were harvested on day 4 p.i., and cell lysates were incubated with StrepTactin-Sepharose. Eluted proteins (bound) were analyzed by immunoblotting using antibodies against the HA tag, pUL56, pUL89, or pUL52. Input lanes contained whole-cell lysate before pulldown with StrepTactin-Sepharose. (C) HD-UL51-SF-infected fibroblasts were lysed on day 4 p.i., and immunoprecipitation was performed with either the pUL56- or the pUL89-specific antibody. Eluted proteins were subjected to immunoblotting (WB) with antibodies directed to the indicated proteins.