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HCMV UL51 mutants generated in this study
| HCMV mutant | UL51 disrupted? | Ectopic UL51 taga | EGFP fluorescence | Growth |
|---|---|---|---|---|
| pHG-ΔUL51 | Yes | + | − | |
| pHG-UL51-CHA | Yes | HA tag (C) | + | − |
| pHG-UL51-CHA-DN | No | HA tag (C) | + | + |
| pHG-UL51-NHA | Yes | HA tag (N) | + | + |
| pHD-ΔUL51 | Yes | − | − | |
| pHD-UL51-NHA | Yes | HA tag (N) | − | + |
| pHD-UL51-SF | Yes | Strep-Flag–HA tag (N) | − | + |
Indicated in parentheses is the placement of the tag, i.e., at the C or N terminus.
