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GFP-TMEM106B overexpression results in LAMP1-positive vacuoles in N2A cells. (A) GFP-TMEM106B expression in N2A cells results in enlarged vacuoles that are rimmed by TMEM106B and LAMP1. (B) GFP-TMEM106B-induced vacuoles are EEA1 negative. (C) Colocalization of PGRN with GFP-TMEM106B in some of the vacuoles. Scale bar: 10 µm (2 µm in the inset).
