Table 3.

In vitro expression of human wild-type or mutant CYP26C1 constructs in Cos-1 cells^a

	Mean aqueous radioactivity (3H-retinoic acid; counts per minute)	Mean aqueous radioactivity—pCMV6-XL5 (empty vector) (3H-retinoic acid; counts per minute)	Radioactivity as % of CYP26C1/wild type
pCMV6-XL5 (empty vector)	3130.28 ± 41.67	0	–
CYP26C1 wild type	10 375.46 ± 8.13	7245.18	100%
CYP26C1/ c.844_851dupCCATGCA	3421.78 ± 172.96	291.5	4%
CYP26C1/c.1433A>G	3167.24 ± 122.0	36.96	0.01%

^aEmpty pCMV6-XL5 vector, CYP26C1 wild type, CYP26C1/ c.844_851dupCCATGCA and CYP26C1/c.1433A>G were transfected into Cos-1 cells. CYP26C1 enzyme activity was determined by the conversion of radiolabeled, released ³H-retinoic acid (counts per minute) as described in the Materials and Methods section. The data in the second column represent the mean ± standard error of the mean of triplicate samples of a representative experiment. The values in the third column have been corrected for background, based on subtraction of the background counts per minute obtained from the transfection of the empty pCMV6-XL5 vector.