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Haploinsufficiency in the primary immune cells from heterozygous IFNGR2 individuals. (A) Naive CD4+ T cells, (B) IL-4-producing T helper cells, (C) monocytes (D) MDMs generated in the presence of either M-CSF + IL-4 or GM-CSF + TNF-α were stimulated by incubation with IFN-γ, IFN-α or IL-27 for 15 min. Western blots were carried out to evaluate STAT1 phosphorylation. These experiments were carried out at least twice.
