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. 2013 Feb;33(3):485–497. doi: 10.1128/MCB.01189-12

Fig 7.

Fig 7

HBx alters p53 occupancy by enhancing PCAF-mediated acetylation at p53 Lys320. (A) Immunoblots of Ac-p53 Lys320, -373, and -382 expression of control and HBx THLE-3 cells using site-specific modification antibodies. Immunoblots of total p53, GAPDH, and HBx expression are also shown. (B) ChIP-qPCR using Ac-p53 Lys320-specific antibody performed on HBx and control THLE-3 cells. (C) p53AIP1 expression measured by qPCR in HBx and control Hep3B cells transfected with wild-type p53, acetylation mimic K320Q, or nonacetylatable K320R. An immunoblot of p53 expression is shown. (D) Immunoblot of Ac-p53 Lys320 expression in HDAC1-specific and control siRNA-treated THLE-3 cells. Immunoblots of HDAC1 and GAPDH expression are shown. (E) ChIP-qPCR using PCAF-specific antibody performed on HBx and control THLE-3 cells. (F) p53AIP1 expression measured by qPCR in PCAF-specific or control siRNA-treated HBx and control THLE-3 cells. Immunoblots of Ac-p53 Lys320 and PCAF expression are shown. All error bars show standard errors of the mean from triplicate experiments.