Table 1.
Table of primers and respective DNA sequences
| Primer use and name | Orientationa | DNA sequence (5′–3′)b |
|---|---|---|
| ChIP-qPCR | ||
| p53AIP1 promoter p53 RE | F | TCAGGGTGAGATGTCTTATC |
| R | CACAGGCAGAATTGTCATTT | |
| p53AIP 1 intron 1 p53 RE | F | CTCTTGCTAATGCCAGCCTG |
| R | GCATCAGGAAGTTCATCTCG | |
| RT-qPCR: p53AIP1 | F | CACCCAGTCACAGCAGCACA |
| R | CAGAGGAAGATCCCATCCAG | |
| Cloning and mutagenesis | ||
| Wild-type construct | F | AGGAACGATGGAATCAGAGTCAC |
| R | GCAGCAGCAAGGCACCATCATG | |
| Mutant promoter p53 RE | F | TAGaATtTCTGAAAGTTGGCAAgtgGTAAAAAGGC |
| R | TTACcacTTGCCAACTTTCAGAaATtCTATTCCG | |
| Mutant intron 1 p53 RE | F | CTCTaTTaCCCGGGtactTCGAGATGAAC |
| R | CATCTCGAagtaCCCGGGtAAtAGAGGAG | |
| Mutant YY1 RE | F | TACAATAAAAgacaGcCTAGGGAGAAATTACCCAGCAC |
| R | TTCTCCCTAGgCtgtcTTTTATTGTAGAGAATGGAAACCTG | |
| Mutant GATA-1 RE | F | GATGTCTTcTCCGGTTAACTGC |
| R | GCAGTTAACCGGAgAAGACATC | |
| Mutant Sp1 RE | F | CCTCATCttGCCCCCTGCAC |
| R | GTGCAGGGGGCaaGATGAGG | |
| p53 K320Q mutant | F | CAGCCAAAGcAGAAACCACTGGATGG |
| R | TGGTTTCTgCTTTGGCTGGGGAGAGG | |
| p53 K320R mutant | F | CAGCCAAAGAgGAAACCACTGGATGG |
| R | TGGTTTCcTCTTTGGCTGGGGAGAGG |
a
F, forward; R, reverse.
b
Lowercase letters denote introduced mutations.