Table 3.
Oligonucleotides used to construct plasmids
| Oligonucleotide | Plasmida | Sequence (5′→3′)b | Reference |
|---|---|---|---|
| ENV7-HA FP | pSMG17 | CTACTTTTTACAACAAATCTAGAATTCCTGCAGCCCGGGGGATCCatgatttctattgtattggaa | This study |
| ENV7-HA RP | CCCGCATAGTCAGGAACATCGTATGGGTAAAAGATGCGGCCCAGAtcagtgtctaaatcttgtaaaag | This study | |
| GFP-ENV7 FP | pSMG18 | GCTGCTGGGATTACACATGGCATGGATGAACTATACAAATCTAGAatgatttctattgtattggaa | This study |
| GFP-ENV7 RP | TCTTTTCGTCTTAGCGTTTCTACAACTATTTCCTTTTTATCAAgtgtctaaatcttgtaaaag | This study | |
| ENV7-GFP FP | pSMG19 | ATTCAAATGTAATAAAAGTATCAACTCGAGGTCGACGGTATCGATatgatttctattgtattggaa | This study |
| ENV7-GFP RP | AAGAATTGGGACAACTCCAGTGAAAAGTTCTTCTCCTTTACTCATtctagaagtgtctaaatcttgtaaaag | This study | |
| ENV7-6×HIS FP | pSMG24 | CTACTTTTTACAACAAATCTAGAATTCCTGCAGCCCGGGGGATCCatgatttctattgtattggaa | This study |
| ENV7-6×HIS RP | GTACATAGGAAATTTTAAACGGTATACAAGTACGTAAAAAAGGTCaatgatgatgatgatggtgagtgtctaaatcttgtaaaagatttaataattggc | This study | |
| E269AENV7-HA mutant FP | pSMG25 | gccatatactccaccagcacttctgaacttgaaattaaatcagg | This study |
| E269AENV7-HA mutant RP | cctgatttaatttcaagttcagaagtgctggtggagtatatggc | This study | |
| E269D ENV7-HA mutant FP | pSMG26 | gccatatactccaccagaccttctgaacttgaaattaaatcagg | This study |
| E269D ENV7-HA mutant RP | cctgatttaatttcaagttcagaaggtctggtggagtatatggc | This study | |
| E269S ENV7-HA mutant FP | pSMG27 | gccatatactccaccaagccttctgaacttgaaattaaatcagg | This study |
| E269S ENV7-HA mutant RP | cctgatttaatttcaagttcagaagtgctggtggagtatatggc | This study | |
| K69-70R ENV7-HA mutant FP | pSMG28 | gaattatacgcactaaggaggattatttgtcctag | This study |
| K69-70R ENV7-HA mutant RP | ctaggacaaataatcctccttagtgcgtataattc | This study | |
| K69-70M ENV7-HA mutant FP | pSMG29 | gaattatacgcactaatgatgattatttgtcctag | This study |
| K69-70M ENV7-HA mutant RP | ctaggacaaataatcatcattagtgcgtataattc | This study | |
| C13-15S ENV7-HA mutant FP | pSMG30 | gtattggaattgttccagaacttgtcctcctctcgcggattttcc | This study |
| C13-15S ENV7-HA mutant RP | ggaaaatccgcgagaggaggacaagttctggaacaattccaatac | This study | |
| GFP-C13-15S ENV7 FP | pSMG31 | gtattggaattgttccagaacttgtcctcctctcgcggattttcc | This study |
| GFP-C13-15S ENV7 RP | ggaaaatccgcgagaggaggacaagttctggaacaattccaatac | This study | |
| ENV7-6×HIS (bacterial) FP | pSMG34 | atgccatggcaatttctattgtattgg | This study |
| ENV7-6×HIS (bacterial) RP | atgaagctttcaatgatgatgatgatggtgagtgtctaaatcttgtaa | This study | |
| KpnI/ENV7-FP | pSMG470 | ggggggtacctgacggtcagaaaagattagtcatt | This study |
| ClaI/ENV7-RP | ggggatcgattaatatgggagtgggcagca | This study |
a
Plasmids are listed in Table 2.
b
Vector-specific sequences are capitalized; ENV7-specific sequences are in lowercase. Point mutations are indicated in boldface. Restriction sites are underlined.