Fig 6.

RIP1 forms a complex with FADD in TAK1-KO MEFs in response to TNF-α. (A) WT and TAK1-KO MEFs were stimulated with TNF-α (10 ng/ml), and the cell lysates were immunoprecipitated with an anti-FADD antibody and immunoblotted with anti-RIP1 (top) or anti-FADD (bottom). The cell lysates were immunoblotted with the indicated antibodies (Long Exp, long exposure; Short Exp, short exposure). (B) Reconstitution of TAK1-KO MEFs with TAK1 but not TAK1-K63A blocks FADD and RIP1 interaction in response to TNF-α. TAK1-KO MEFs expressing empty vector (pMX), TAK1, or TAK1-K63A were processed as described for panel A.