Table 1. Effects of OPA1 knockdown on mitochondrial respiration in different metabolic states.
| Respiration rates, O2 nmol/min per 107 cells | ||||
|---|---|---|---|---|
| |
Metabolic state |
Control (scrambled siRNA) |
OPA1 knockdown (OPA1 siRNA) |
Inhibition of respiration by OPA1 knockdown: % inhibition |
| Permeabilized cells (n=6) | V3* | 16.7±1.6 | 13.4±1.0 | 19.1±2.2 |
| V4 | 3.3±0.2 | 3.1±0.3 | 6.4±7.9 | |
| V3u* | 20.2±2.5 | 17.3±1.9 | 14.0±4.5 | |
| Intact cells (n=8) | Vbasal* | 17.2±1.9 | 13.0±1.2 | 21.0±5 |
| V4 | 8.8±6.3 | 6.3±0.5 | 15.7±12.1 | |
| V3u* | 25.0±2.0 | 18.3±1.6 | 26.0±4 | |
Data are means±S.E.M.; n, number of experiments. To determine the percent inhibition, respiration rates for each experiment were pairwise normalized to corresponding rates in the control (defined as 100%)
*Significant at P<0.05 by paired Student's t-test