Figure 7.

Overexpressed Trim17 decreases the protein level of Mcl-1. (a) Neuro2A cells were transfected with 0.3 μg pCI-Mcl-1(WT) together with the indicated amounts of pCI-Trim17 or pCI-Trim17(ΔRING). Twenty four hours after transfection, cell lysates were analyzed by western blot using antibodies against Trim17, Mcl-1 and actin. (b) Neuro2A cells were transfected for 24 h with the different forms of Mcl-1 (0.5 μg of each plasmid), together with 1 μg pCI-Trim17 when indicated. Then, cells were treated for 5 h with 25 μM MG-132 (MG), or 10 μM AR-A014418 and 10 μM SP600125 (AR+SP), before cell harvesting, when indicated. Cell lysates were analyzed by western blot using antibodies against Trim17, Mcl-1 and actin