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. 2012 Aug 31;20(2):235–247. doi: 10.1038/cdd.2012.113

Figure 2.

Figure 2

ROC1 silencing induced G2–M cell cycle arrest and p21-dependent cell senescence. (a and b) ROC1 silencing induced G2–M cell cycle arrest in HepG2 (a) and Huh7 (b) cells. Cells were transfected with siControl or siROC1 for 48–120 h, and subjected to PI staining and FACS analysis to determine cell cycle profile. (c and d) ROC1 silencing induced cell senescence in HepG2 (c) and Huh7 (d) cells. Cells were transfected with siControl or siROC1 for 120 h and subjected to cellular morphological observation (top left panels), SA-β-gal staining (bottom left panels) and quantification of positively stained cells (right panels). (e and f) ROC1 silencing-induced cell senescence is p21-dependent in HepG2 (e) and Huh7 (f) cells. The HepG2 and Huh7 cells transfected with siRNAs for 96 h, and were subjected to IB analysis of expression of proteins indicated (left panels) and senescence analysis with SA-β-gal staining (right panels). The results were presented as mean value±S.E. from three independent experiments, ***P<0.001