FIG. 1.

Estrogen treatment at the time of OVX reduces diet-induced obesity. A: OVX led to increased body weight with HFD. Mice treated with E2 at the time of OVX did not gain weight with HFD. B: Adiposity was increased with HFD in sham mice and after OVX. Adiposity was reduced with E2 treatment. Letter B indicates baseline before HFD-feeding. Letter E indicates end point after HFD. C: Plasma cholesterol levels. D: Plasma triglyceride levels. E: HFD increased fasting insulin levels in sham and OVX mice, and E2 treatment reduced plasma insulin. F: Fasting glucose levels were not different between groups. *P < 0.05. For panel B, differences from baseline were defined by Student t test. For C–F, differences between groups were determined by ANOVA followed by Tukey post hoc tests.