Table 1. PFAMs that target plant polymers, identified in metagenomes from high-Arctic peatlands of Svalbard.
| S2a | S2b | K1a | K1b | K1c | |
|---|---|---|---|---|---|
| Cellulases | |||||
| GH5 | 4 (2.3) | 4 (1.8) | 4 (2.1) | 8 (3.9) | 18 (5.8) |
| GH6 | 2 (1.2) | 0 (0) | 1 (0.5) | 1 (0.5) | 2 (0.7) |
| GH7 | 0 (0) | 1 (0.5) | 0 (0) | 1 (0.5) | 0 (0) |
| GH9 | 1 (0.5) | 2 (0.9) | 4 (2.1) | 1 (0.5) | 6 (2.0) |
| GH44 | 2 (1.2) | 2 (0.9) | 3 (1.6) | 2 (1.0) | 0 (0) |
| GH45 | 1 (0.6) | 0 (0) | 0 (0) | 0 (0) | 1 (0.3) |
| GH48 | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| Sum | 10 (5.9) | 9 (4.1) | 12 (6.3) | 13 (6.3) | 27 (8.8) |
| Debranching enzymes | |||||
| GH51 | 5 (2.9) | 8 (3.6) | 3 (1.6) | 5 (2.4) | 13 (4.2) |
| GH54 | 1 (0.6) | 3 (1.4) | 0 (0) | 0 (0) | 0 (0) |
| GH62 | 0 (0) | 0 (0) | 1 (0.5) | 0 (0) | 0 (0) |
| GH67 | 2 (1.2) | 1 (0.5) | 0 (0) | 0 (0) | 2 (0.7) |
| GH78 | 1 (0.6) | 2 (0.9) | 2 (1.1) | 10 (4.9) | 15 (4.9) |
| Sum | 9 (5.3) | 14 (6.4) | 6 (3.1) | 15 (7.3) | 30 (9.7) |
| Endohemicellulases | |||||
| GH8 | 0 (0) | 0 (0.0) | 1 (0.5) | 0 (0) | 2 (0.7) |
| GH10 | 2 (1.2) | 6 (2.7) | 1 (0.5) | 5 (2.4) | 7 (2.3) |
| GH11 | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0) | 1 (0.3) |
| GH12 | 0 (0.0) | 1 (0.5) | 0 (0.0) | 0 (0) | 0 (0) |
| GH26 | 4 (2.3) | 4 (1.8) | 0 (0) | 1 (0.5) | 12 (3.9) |
| GH28 | 0 (0.0) | 1 (0.5) | 4 (2.1) | 2 (1.0) | 8 (2.6) |
| GH53 | 0 (0.0) | 3 (1.4) | 3 (1.6) | 2 (1.0) | 4 (1.3) |
| Sum | 6 (3.5) | 15 (6.8) | 9 (4.7) | 10 (4.9) | 34 (11.0) |
| Oligosaccharide-degrading enzymes | |||||
| GH1 | 9 (5.3) | 10 (4.6) | 6 (3.1) | 14 (6.8) | 20 (6.5) |
| GH2 | 5 (2.9) | 0 (0.0) | 4 (2.1) | 4 (1.9) | 15 (4.9) |
| GH3 | 5 (2.9) | 9 (4.1) | 6 (3.1) | 9 (4.4) | 18 (5.8) |
| GH29 | 5 (2.9) | 4 (1.8) | 4 (2.1) | 11 (5.3) | 13 (4.2) |
| GH35 | 2 (1.2) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 3 (1.0) |
| GH38 | 0 (0.0) | 0 (0.0) | 1 (0.5) | 3 (1.5) | 4 (1.3) |
| GH39 | 2 (1.2) | 5 (2.3) | 1 (0.5) | 2 (1.0) | 7 (2.3) |
| GH42 | 3 (1.8) | 2 (0.9) | 2 (1.1) | 6 (2.9) | 11 (3.6) |
| GH43 | 8 (4.7) | 7 (3.2) | 5 (2.6) | 8 (3.9) | 10 (3.2) |
| GH52 | 0 (0.0) | 0 (0.0) | 0 (0.0) | 2 (1.0) | 0 (0.0) |
| Sum | 39 (23) | 37 (17) | 29 (15) | 59 (29) | 101 (33) |
| Phenolic compound-degrading enzymes | |||||
| Laccase | 1 (0.6) | 8 (3.6) | 7 (3.7) | 11 (5.3) | 12 (3.9) |
| Dioxygenase | 2 (1.2) | 8 (3.6) | 3 (1.6) | 2 (1.0) | 5 (1.6) |
| Peroxidase | 4 (2.3) | 5 (2.3) | 3 (1.6) | 8 (3.9) | 1 (0.3) |
| Sum | 7 (4.1) | 21 (9.6) | 13 (6.8) | 21 (10.2) | 18 (5.8) |
| Tot. ass. seq. | 17 067 | 21 980 | 19 104 | 20 581 | 30 834 |
The metagenomic profile is divided into five categories according to the functional role of the protein families in plant polymer degradation, a format previously applied in Allgaier et al. (2010) and Pope et al. (2010). Numbers reflect the number of significant hits (see Materials and methods for details). Shown in brackets are the numbers in 0/000 of the total number of sequences assigned to a PFAM. Tot. ass. seq., the number of sequences that were assigned to a PFAM (See Materials and methods for details). See Supplementary Table S4 for PFAM accession numbers and description of protein families.