Table 1.
Production of caffeic acid and ferulic acid by E. coli strains with pET-T5 and pET-opT5 or pET-T5M and pET-opT5M
| Engineered E. coli strains | 4-Coumaric acid (mg/L) | Caffeic acid (mg/L) | Ferulic acid (mg/L) |
|---|---|---|---|
| pET-T5 |
ND |
42 ± 19a |
- |
| pET-opT5 |
26 ± 8 |
14 ± 2a |
- |
| pET-T5M |
ND |
ND |
28 ± 10b |
| pET-opT5M | ND | ND | 73 ± 15b |
The engineered strains are cultured in an M9 medium containing glucose 15 g/L. Each batch cultivation was done at least in triplicate, and the standard deviations are shown. Different letter codes (a and b) indicate significant differences (a, P = 0.0664; b, P = 0.00226). The code ‘ND’ indicated that something was not detected in our HPLC analysis.