Table 2.
Production of caffeic acid and ferulic acid by engineered tyrosine overproducing E. coli strains
| Engineered tyrosine overproducing E. coli (pAD-AG/ΔtyrR) | 4-Coumaric acid (mg/L) | Caffeic acid (mg/L) | Ferulic acid (mg/L) |
|---|---|---|---|
| pET-TAL |
974 ± 30a |
- |
- |
| pET-opTAL |
805 ± 41a |
- |
- |
| pET-T5 |
307 ± 8 |
150 ± 8b |
- |
| pET-opT5 |
511 ± 69 |
40 ± 16b |
- |
| pET-T5M |
113 ± 12 |
22 ± 3 |
196 ± 26c |
| pET-opT5M | 150 ± 17 | 46 ± 7 | 187 ± 12c |
Each batch cultivation was done at least in triplicate, and the standard deviations are shown. Different letter codes (a to c) indicate significant differences (a, P = 0.0216; b, P = 7.17E-07; c, P = 0.5176).