Figure 6.

IL-11 fails to stimulate osteoclastogenesis even with permissive level of RANKL. (A) BMMs were cultured with rM-CSF (10 ng/ml) plus RANKL (10 ng/ml) with or without IL-11 (10 ng/ml) for 6 days in tissue culture dish. The cultures were then stained for TRAP activity. Each condition had three replicates (wells) and was repeated 4 times. A representative area of the culture from each condition is shown. (B) BMMs on bone slices were treated with the same conditions, but cultured for 9 days and resorption pits were then visualized by SEM. Magnification by SEM was 200x. Each resorption assay had two replicates (bone slices) Quantification of the bone resorption assays is shown, bars shown averaged ± S.E. *, p < 0.0001 (C) BMMs were cultured with rM-CSF (10 ng/ml) plus RANKL (100 ng/ml) as control or rM-CSF (10 ng/ml) with sub-optimal levels of RANKL (10 ng/ml) with or without IL-11 (200 ng/ml) for 6 days in a tissue culture dish. The cultures were then stained for TRAP activity. Each condition had three replicates (wells) and was repeated 3 times. A representative area of the culture from each condition is shown.