Table 1.
PPOX probe mix
| Probe name | Sizea | 5′half hyb sequenceb | 3′half hyb sequencec | SD | N1 | N2 | Fam A | Fam E |
|---|---|---|---|---|---|---|---|---|
| GABRA4d |
84 |
CAGCCTGTTGTCATAACCATCG |
AGCAAACTGTCCAGGATGCG |
0.20 |
nd |
nd |
nd |
nd |
| RELNe |
87 |
CAGCATTACGGAATGAAGGTCA |
CCACAAGAAGTGGCTTCACAACC |
0.04 |
1.02 |
0.99 |
0.98 |
0.96 |
| PPOXex1 |
90 |
GGGAGTAGCGGATTTGAAGCACTT |
GTTGGCCTACAGAGGTGTGGCAAG |
0.03 |
0.94 |
1.00 |
1.07 |
1.01 |
| PPOXex7v3 |
93 |
CTGAGCGCTGGAGCCAGTGGTCACTT |
CGTGGAGGTCTAGAGATGTTGCCTC |
0.04 |
1.00 |
0.99 |
1.03 |
0.55 |
| PPOXex5v5 |
96 |
GCAAAGAGCCTGATGAGACTGTGC |
ACAGTTTTGCCCAGCGCCGCCTTGGACCTG |
0.03 |
1.04 |
1.00 |
0.57 |
0.60 |
| PPOXex6 |
99 |
GTGTTTGCAGGCAACAGCCGTGAGCTCA |
GCATCAGGTCCTGCTTTCCCAGTCTCTTC |
0.03 |
1.02 |
1.02 |
0.59 |
0.58 |
| PPOXex3 |
102 |
GCTCCGTTCGAGGCCCTAATGGTGCTATCT |
TTGAGCTTGGACCTCGGGGAATTAGGCCAG |
0.02 |
1.00 |
0.93 |
1.04 |
1.11 |
| CLDN16e |
105 |
GACACAAGGGTGTAAAATGCACG |
TTTCAGGGTGTGTTTGCATATGATTTAATCAATCAGTATG |
0.04 |
1.00 |
1.02 |
1.01 |
1.00 |
| PPOXex9 |
108 |
GTGCCATCACTGCAGTGTCTGTAGCTGTGGTGA |
ATCTGCAGTACCAAGGAGCCCATCTGCCTGTCC |
0.03 |
1.05 |
0.99 |
1.02 |
0.58 |
| PPOXex11 |
111 |
CTGTTTCAACAGCGGGCCCAGGAAGCAGCTGCTA |
CACAATTAGGACTGAAGGAGATGCCGAGCCACTGC |
0.03 |
1.04 |
1.04 |
0.99 |
1.22 |
| PPOXex4 |
114 |
CATGCCCTACCCACTGGCCTCAGGTAACACCAGCA |
CCTCCGCTCCTTTTACTGTGCCCTCATCCTCATATGC |
0.02 |
1.05 |
1.03 |
1.06 |
1.16 |
| PPOXex8 |
117 |
CTCATTTTCTGGGTCTCTCAAATGTTTTCATGCTCTC |
AGGTATCTCTAAGGGACAGCAGTCTGGAGGCTGACCAC |
0.04 |
1.06 |
1.03 |
0.98 |
0.56 |
| PPOXex12 |
120 |
CTGGCAAAAACTAGGTAAGTTGGGAAAACAGCTGGGCT |
GAGGAGGGCCAAGGACATCAGACCCCCAGCTAAAACATTC |
0.03 |
1.06 |
1.09 |
0.99 |
1.16 |
| PPOXex10 |
123 |
CTGACGCATGAATGTCCTTCTCTCCAGGGATTTGGACATT |
TGGTGCCATCTTCAGAAGATCCAGGAGTCCTGGGAATCGTG |
0.03 |
1.06 |
1.09 |
0.93 |
1.16 |
| PPOXex13 |
126 |
GCTGTTAATGACTGTATAGAGAGTGGGCGCCAGGCAGCAGTCA |
GTGTCCTGGGCACAGAACCTAACAGCTGATCCCCAACTCTC |
0.03 |
1.08 |
1.03 |
1.04 |
1.13 |
| RB1ex23e |
129 |
GTCACCAATACCTCACATTCCTCGAAGCCCTTACAAGTTTCCT |
AGTTCACCCTTACGGATTCCTGGAGGGAACATCTATATTTCACC |
0.02 |
1.00 |
1.02 |
0.98 |
1.11 |
| PPOXex2v4 |
132 |
CCTAAGGTGAGTGCTCCACTTGTGCCAGAGGGAGCTTCATTTAATGC |
TCTTCCCATTTCCATCAAAAGCTAGATGGATCCTGGCCCTCTG |
0.02 |
1.01 |
1.00 |
1.09 |
1.08 |
| SRY | 135 | CAGTGCAAAGGAAGGAAGAGCTTCTCCGGAGAGCGGGAATATTCT | CTTGCACAGCTGGACTGTAATCATCGCTGTTGAATACGCTTAACATAG | nd | nd | nd | nd | nd |
aSize of the ligaton product in bp.
bThe 5′ half-probes are preceded by the universal tag sequence GGGTTCCCTAAGGGTTGGA.
cThe 3′ half-probes are followed by the universal tag sequence CTAGATTGGATCTTGCTGGCAC and are phosphorylated at the 5′ end.
dPilot probe.
eReference probes.
SD standard deviation.
Nd not determined.