Figure 2. Concentration-dependent effects of CXB analogues on Kv7.2/7.3 expressed in HEK293 cells.
The currents were recorded using the protocol shown at the left top. A–C, Representative traces of Kv7.2/7.3 current responding to the different concentrations of UMC, DMC and CXB were shown. The dotted line indicates the zero current level. D, The concentration-response relationships for activation of Kv7.2/7.3 currents by CXB analogues were fitted with the logistic function. Folds of currents increased at – 30 mV were plotted against drug concentrations. The EC50 value is 22.7 ± 3.0 µM for UMC, 4.5 ± 0.7 µM for CXB, and 2.5 ± 0.2 µM for DMC. n = 4–6.