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. 2013 Jan 24;8(1):e54797. doi: 10.1371/journal.pone.0054797

Figure 5. CXB analogues attenuate nocifensive behavior induced by BK, thermal and mechanical stimuli.

Figure 5

A, Effects of CXB, UMC) DMC and RTG on BK-induced nocifensive behavior. 100 µM CXB, UMC, DMC or RTG was injected 5 min before the injection of BK (200 µM) plus solvent or the corresponding compounds into hind paw in volume of 50 µl in each case. The second injection was done into the same site. The time of animals spent licking, biting, lifting and flitching during 30 min after injection were recorded and shown. n = 10 for each group. *P <0.05, **P <0.01, compared with the BK plus solvent group. B, Effects of CXB, UMC, DMC and RTG on mechanical-induced nocifensive behavior. 100 µM CXB, UMC, DMC, RTG or solvent was were injected into rat hind paw in volume of 50 µl. 8 min later, paw withdrawal thresholds (g) were measured using calibrated Von Frey filaments applied to the plantar surface of the injected paw. n = 10. *P <0.05, ***P <0.001, compared with the solvent group. C, Effects of CXB, UMC, DMC and RTG on thermal-induced nocifensive behavior. 100 µM CXB, UMC, DMC, RTG or solvent was were injected into rat hind paw in volume of 50 µl. 8 min later, the injected hind paw was subjected to radiant heat from underneath the glass floor with a high-intensity lamp bulb, and paw withdrawal latency was measured and presented. n = 10. *P <0.05, ***P <0.001, compared with the solvent group.