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. 2013 Jan 24;9(1):e1003114. doi: 10.1371/journal.ppat.1003114

Figure 2. Serum components, specifically C3, have an inhibitory effect on pro-inflammatory cytokine production upon Schu S4 infection of human macrophages.

Figure 2

(A–C) hMDMs were infected with Ft Schu S4 at an MOI of 50∶1 in RHH or RHS with 10% autologous serum, C3-depleted serum (C3d) or C3-repleted serum (C3r) for 1 h; or (D–F) hMDMs were infected with Ft Schu S4 (Ft) or F. novicida (Fn) at an MOI of 50∶1 in RHH or RHS with 10% autologous serum. Extracellular bacteria were killed with 50 µg/ml gentamycin at 37°C for 30 min. Media were replenished and cell-free culture supernatants were collected at 16 hrs post infection. TNFα, IL-6 and IL-1β concentrations were measured by ELISAs. Uninfected resting cells (R) were included as a control. Data are representative of at least 3 independent experiments. The data were analyzed by 1-Way ANOVA and Tukey's Multiple Comparison Test. ** p<0.005.