Table 5. Pancreatic cancer cell conditioned media effects on PDL1 and CTLA4 in immature myeloid cell subsets.
| Control (n = 14) | BxPC3 (n = 6) | Capan1 (n = 8) | MiaPaCa2 (n = 6) | |
| CD14+HLA-DR+PDL+ | 56 (37–73) | 61 (23–82) | 76 (73–92) | 50 (21–76) |
| p-value | 0.249 | 0.036* | 0.686 | |
| CD14 − HLA-DR+PDL+ | 31 (22–36) | 26 (10–50) | 46 (39–53) | 33 (28–45) |
| p-value | 0.753 | 0.017* | 0.173 | |
| CD14 − HLA-DR − PDL+ | 2.7 (0.4–4.8) | 3.3 (0.3–6.3) | 3.8 (1.3–10.6) | 1.7 (1.3–5.8) |
| p-value | 0.753 | 0.108 | 0.345 | |
| Control (n = 12) | BxPC3 (n = 6) | Capan1 (n = 6) | MiaPaCa2 (n = 6) | |
| CD14+HLA-DR+CTLA4+ | 16 (12–23) | 28 (9–54) | 6 (1–8) | 15 (10–20) |
| p-value | 0.345 | 0.028* | 0.500 | |
| CD14 − HLA-DR+CTLA4+ | 13 (4–21) | 4 (2–16) | 4 (1–7) | 10 (2–57) |
| p-value | 0.116 | 0.043* | 0.917 | |
| CD14 − HLA-DR − CTLA4+ | 12.3 (10.0–13.2) | 5.7 (0.5–10.7) | 4.3 (2.9–5.8) | 8.3 (2.9–19.5) |
| p-value | 0.028* | 0.046* | 0.753 |
Healthy PBMC were analysed by flow cytometry after they have been cultured for 4 days in control medium or in pancreatic cancer cell conditioned media. PBMC from 8 donors were cultured in Capan1 conditioned and in their respective control media, while PBMC from 6 donors were cultured in BxPC3 and MiaPaCa2 conditioned media and in their respective control media. In the series of 8 PBMC donors used for Capan1 experiments, CTLA4 data was available for a subset of 6 donors. Median and interquartile ranges (in brackets) of the percentage of CD33+ immature myeloid cells expressing PDL1 or CTLA4 are shown. Only few events among the CD33+CD14+HLA-DR− cell population were obtained (see table 2) and this did not allow an accurate analysis of this subset, which was omitted from the table. The statistical analysis of data (Wilcoxon signed rank test) was made by pairing any conditioned media result with its own control. Asterisks highlight statistical significance.