Figure 3. Antimicrobial activity of CHAPSH3b protein in skim milk.

Milk was inoculated with 10³ CFU/ml of S. aureus Sa9 and incubated in the presence of 1.65 µM CHAPSH3b for 0, 15, 30, 60 and 120 min either at RT or 37°C in: A) raw milk and B) pasteurized milk. Dark grey bars indicate S. aureus Sa9 control culture and light grey bars S. aureus Sa9+ CHAPSH3b. Values, expressed as log CFU/ml, are the means ± standard deviations of two independent experiments. Bars having asterisks are significantly different from the control (***P<0.001). S. aureus detection threshold (<10 CFU/ml).