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. 2013 Jan 24;8(1):e54245. doi: 10.1371/journal.pone.0054245

Figure 3. MTT assay to determine the viability of RAW 264.7 cells.

Figure 3

RAW 264.7 cells were (A) exposed to Type II and Type III mutants of PAO1 for 1 h, or (B) to PAO1 in the presence of rifampicin (Rfp, 40 µg/ml), or gentamicin (Gm, 200 µg/ml). In (C), cells were exposed to different mutant strains of PAO1 containing mutations in pili and flagella. In (D), cells were exposed to muramyldipeptide (MDP, 25 µg/ml) or CpG DNA (5 µg/ml). In all cases cell viability was estimated by measuring MTT activity after 24–48 h as mentioned in text. (***) designates p<0.0003. ns: not-significant.