Figure 3. Effect of blue light on biofilm and motility mediated by A. baylyi ´s photoreceptors in an A. baumannii genetic background.

A. Cells of the ATCC 17978.OR blsA mutant, transformed with plasmids pWH1499, pWH2110, pWH2125 and pWH2129 or the empty pWH1266 vector, were inoculated on the surface of swimming plates. Plates were inspected and photographed after overnight incubation in darkness (D) or the presence of blue light (L) at 24°C. B. The biofilms formed by ATCC 17978.OR blsA mutant, transformed with plasmids pWH1499, pWH2110, pWH2125 and pWH2129 or the empty pWH1266 vector on glass tubes were recorded after static incubation for 96 h at 24°Cby direct visual inspection and staining with crystal violet. C. Quantification of the biofilms of cognate samples shown in B. Error bars show standard error of the mean for 3 different biological replicates (n = 3). OD₅₈₀/₆₀₀, optical density at 580 or 600 nm, respectively. L: light; D: Dark.