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. 2013 Jan 24;8(1):e54886. doi: 10.1371/journal.pone.0054886

Figure 1. Reverse transcription and PCR primers.

Figure 1

A: Oligonucleotides used for priming reverse transcription of pre-rRNA (cDNA synthesis), and forward and reverse PCR primers for amplifying cDNA. B: Specificity of PCR primer sets designed for S. aureus, A. baumannii, P. aeruginosa, and MTBC. Each primer set was used in endpoint PCR conducted on purified DNA from all the four organisms. Key: L, 1 Kb+ DNA ladder; 1, S. aureus DNA; 2, A. baumannii DNA; 3, P. aeruginosa DNA; 4, M. tuberculosis DNA; 5, negative control (water). Lowest three bands of ladder are 100, 200, and 300 bp. A repeat experiment yielded identical results.