Skip to main content
. 2013 Jan 24;8(1):e54886. doi: 10.1371/journal.pone.0054886

Figure 5. Ratiometric pre-rRNA analysis of A. baumannii cells in serum by using a rapid semi-automated approach.

Figure 5

Serum-incubated cells were plated to quantify viable CFU/mL, then serially diluted in serum. Viable CFU/mL values (X-axis) were subsequently calculated from plating results. To initiate nutritional stimulation, dilutions in serum were further diluted 1∶10 into TSB or PBS (stimulated and non-stimulated, respectively). After 90 minutes, pre-rRNA was quantified by one-step probe-based q-RT-PCR as described in text. Values are means and SDs of ΔCt values (non-stimulated minus stimulated) from two replicates of each dilution. Control samples with no bacteria (0 CFU/mL) yielded no RT-qPCR results, and therefore could not be plotted as ΔCt values. Reaction efficiency could not be calculated for this experiment, because no standard curve was used. A replicate experiment (Figure S3) yielded similar results, showing a limit of detection of ≤56 viable CFU/mL.