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. 2013 Jan 24;8(1):e54692. doi: 10.1371/journal.pone.0054692

Figure 6. Inhibiting Rho kinase blocked proNGF-induced p38/JNK MAPK activation.

Figure 6

A,B. WB analysis of rat retinal lysate showed 1.6- and 1.8-fold increase in the phosphorylation of p38MAPK and JNK in rats electroporated with proNGF as compared with those electroporated with GFP (n = 4–6). C,D. WB analysis of RGC-5 lysate showed 2.4 and 1.9-fold increase in the phosphorylation of p38MAPK and JNK, respectively in RGC-5 cells treated with proNGF as compared with the controls (n = 4). Treatment of rats or RGC-5 with Y27632 blocked all these effects in rats and media treated with proNGF and did not affect the control groups. * = significant difference as compared with the rest of the groups at p<0.05. C, control.