Table 3. Comparison of theoretical and experimental specificity data for mAb JAA-F11 with glycans containing the TF motif present in the CFG v4.0 glycan array.
ID | Glycan Sequence | Theoretical | Experimental RFUa | ||
Clash Scoreb | Sp8c | Sp14 | Sp0 | ||
1 | Galβ1-3GalNAcα | 0 | 98 | 0 | ---d |
2 | Neu5Acβ2-6(Galβ1-3)GalNAcα | 0 | 81 | --- | --- |
3 | Neu5Acα2-6(Galβ1-3)GalNAcα | 0.1 | 78 | 0 | --- |
4 | Galβ1-4GlcNAcβ1-6(Galβ1-3)GalNAcα | 0 | 52 | 0 | --- |
5 | GlcNAcβ1-6(Galβ1-3)GalNAcα | 0 | 51 | 0 | --- |
6 | Neu5Acα2-3Galβ1-4(Fucα1-3)GlcNAcβ1-6(Galβ1-3)GalNAcα | 0 | --- | 0 | --- |
7 | Neu5Acα2-3Galβ1-4GlcNAcβ1-6(Galβ1-3)GalNAcα | 0 | --- | 0 | --- |
8 | (3S)Galβ1-3GalNAcα | 2.8 | 0 | --- | --- |
9 | Fucα1-2Galβ1-3GalNAcα | 7.6 | 0 | 0 | --- |
10 | GlcNAcβ1-2Galβ1-3GalNAcα | 11.6 | 0 | --- | --- |
11 | GlcNAcβ1-3Galβ1-3GalNAcα | 12.4 | 0 | --- | --- |
12 | 6S(Neu5Acα2-3Galβ1-3)GalNAcα | 17.3 | 0 | --- | --- |
13 | Neu5Acα2-3Galβ1-3GalNAcα | 17.4 | 0 | 0 | --- |
14 | Neu5Acα2-6(Neu5Acα2-3Galβ1-3)GalNAcα | 17.6 | 0 | 0 | --- |
15 | KDNα2-3Galβ1-3GalNAcα | 13.8 | --- | 0 | --- |
16 | Neu5Acα2-3Galβ1-4GlcNAcβ1-6(Neu5Acα2-3Galβ1-3)GalNAcα | 17.4 | --- | 0 | --- |
17e | Neu5Acα2-3Galβ1-4(Fucα1-3)GlcNAcβ1-6(Neu5Acα2-3Galβ1-3)GalNAc | 17.5 | --- | 0 | --- |
18e | GlcNAcα1-4Galβ1-3GalNAc | 6.1 | --- | 0 | --- |
19e | Galβ1-3GalNAcα1-3(Fucα1-2)Galβ1-4GlcNAc | 5.1 | --- | --- | 0 |
20e | Galβ1-3GalNAcα1-3(Fucα1-2)Galβ1-4Glc | 5.3 | --- | --- | 0 |
21 | Fucα1-2Galβ1-3GalNAcα1-3(Fucα1-2)Galβ1-4GlcNAcβ | 12.6 | --- | --- | 0 |
22 | Fucα1-2Galβ1-3GalNAcα1-3(Fucα1-2)Galβ1-4Glcβ | 12.8 | --- | --- | 0 |
23 | GalNAcα1-3(Fucα1-2)Galβ1-3GalNAcα1-3(Fucα1-2)Galβ1-4GlcNAcβ | 26.3 | --- | --- | 0 |
Normalized RFUs averaged over all protein concentrations (0.1, 5 and 200 μg/mL) and over multiple values for the same glycan, when present on the CFG array, see Methods. bRelative van der Waals overlap, see Methods. cSpacers, Sp0: –(CH2)2NH2; Sp8: –(CH2)3NH2; Sp14: -threonine. dNot present on glycan array. eReducing anomeric configuration undefined on the CFG array, α-configuration assumed for the CCG analysis.