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. 2013 Jan 24;8(1):e54192. doi: 10.1371/journal.pone.0054192

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© 2013 Kwon et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Immunohistochemistry staining of mouse caput (A, B, C) and cauda (D, E, F) spermatozoa. B and E are merged images of acrosome (Lectin PNA conjugated Alex 647, red) and nucleus (DAPI, blue). C and F are merged image of AVPR2, acrosome and nucleus. Arrows directed AVPR2 (A, C, D, and F). Images obtained using Nikon TS-1000 microscope with NIS Elements image software (Nikon, Japan). Bar = 10 µm.