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. 2013 Jan 24;8(1):e54631. doi: 10.1371/journal.pone.0054631

Figure 6. Comparison of cell infection using pre-mixed vAcBacGFP and vAcBacDsRed with infections using sequentially added viruses.

Figure 6

A. Flow cytometry data for protein expression at 2 days post infection. Single infection: Sf9 cells were infected individually with vAcBacGFP and vAcBacDsRed then mixed and used as the reference to gate the two-colored and single-colored cell subsets; Pre-mixed: vAcBacGFP and vAcBacDsRed viruses were mixed at a ration of 1∶1, stored at 4°C for 1 day and then used to infect Sf9 cells; G0R: 1 MOI of vAcBacDsRed was applied to Sf9 cell monolayer, mixed well and held for 1 min, before adding 1 MOI of vAcBacGFP; R0G: 1 MOI of vAcBacGFP was applied to Sf9 cell monolayer, mixed well and held for 1 min, before adding 1 MOI of vAcBacDsRed. B. Comparison of the fluorescent cell populations. Bars reflect the flow cytometry data shown in A.