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. 2013 Jan 24;8(1):e54590. doi: 10.1371/journal.pone.0054590

Figure 2. Progesterone alleviated demyelination in cuprizone-induced mouse corpus callosum and cortex by LFB, MBP staining and electron microscopy.

Figure 2

Luxol fast blue (LFB) staining is shown in N+Veh (A), N+P (B), CPZ+Veh (C) and CPZ+P mice (D). (E) Statistical analysis of the LFB staining results according to the relative optical density. Bar = 100 µm. MBP-stained myelin is shown in the corpus callosum of N+Veh (F), N+Veh (G), CPZ+Veh (H) and CPZ+P group (I). (J) Statistical analysis by MBP staining according to the relative optical density. MBP-stained myelin is also shown in the cortex of N+Veh (K), N+Veh (L), CPZ+Veh group (M) and CPZ+P group (N). (O) Statistical analysis by MBP staining according to the relative optical density. Bar = 50 µm. Myelin was observed in the corpus callosum of N+Veh (P), N+Veh (Q), CPZ+Veh group (R) and CPZ+P treated group (S) by electron microscopy. (T) Statistical analysis by electron microscopy. Bar = 1 µm in P, Q, R and S. *CPZ+P vs. CPZ+Veh group, P<0.05; **CPZ+Veh vs. N+Veh group, P<0.01.