Skip to main content
. 2013 Jan 24;8(1):e54491. doi: 10.1371/journal.pone.0054491

Figure 6. 14-3-3ζtg mice are protected against seizure-induced neuronal death in vivo and in vitro.

Figure 6

(A) Representative FJB and TUNEL staining for wt and 14-3-3ζtg mice 72 h after status epilepticus for the CA1, CA3 and hilar regions. Scale bar, 120 µm. (B) Semi-quantification of seizure damage and neuron survival (NeuN counts) for wt and 14-3-3ζtg mice (n = 6-10 per group). (C) Primary cultures of hippocampal neurons from wt and 14-3-3ζtg mice were treated with kainic acid and then cell death determined as percentage propidium iodide (PI) positive. (Panels above) Representative photomicrographs of PI-stained neurons 24 h after KA treatment. Scale bar, 25 µm. Graph shows reduced cell death in 14-3-3ζtg mice (n = 3 per group). *p<0.05; **p<0.01; ***p<0.001 compared to wt.