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. 2013 Jan 24;8(1):e54111. doi: 10.1371/journal.pone.0054111

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© 2013 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The scatter plot shows the expression levels of known miRNAs in HepG2 and HepG2/DOX cells. Blue dots: equally expressed miRNAs between HepG2 and HepG2/DOX; Red dots: miRNAs in HepG2/DOX were up-expressed compared to HepG2 (adjusted P<0.05); Green dots: miRNAs in HepG2/DOX were down-expressed compared to HepG2 (adjusted P<0.05). (B,C) The validation of selected up- and down-expressed known miRNAs indicated that the results from deep sequencing were generally agreed well with the qRT-PCR results. (D) The scatter plot shows the expression levels of novel miRNAs in HepG2 and HepG2/DOX cells. (E) The validation of selected novel miRNAs indicated that the results from deep sequencing were generally agreed well with the qRT-PCR results.